Palm Tocotrienols Mixture Supplementation Was As Effective As Estrogen Therapy in Alleviating Oxidative Stress Induced By Ovariectomy in Animal Model


Ahmad Nazrun Shuid, Norazlina Mohamed, Khairunnuur Fairuz Azman and Ima Nirwana Soelaiman


Traditional & Complementary Medicine Exhibition 2007 (TCME 2007), Putra World Trade Centre (PWTC), Kuala Lumpur, Malaysia




Tocotrienol, oxidative stress, menopause


The objective of this study is to assess the ability of palm tocotrienols mixture to prevent oxidative stress induced byovariectomy in rat model. The efficacy of palm tocotrienols mixture in reducing oxidative stress were compared tosubcutaneous injections of estrogen, the hormone replacement therapy usually given post-menopause. 32 female Wistarrats aged 3 months were divided into 4 groups with 8 rats in each group. Group 1(Sham): rats were sham-operated. Group 2(OVX) rats were ovariectomised and given oral gavages of vehicle (olive oil). Group 3 (OVX+PTT) rats were ovariectomisedand given oral gavages of 60 mg/kg rat weight of palm tocotrienols mixture. Group 4 (OVX+EST) rats were ovariectomisedand injected subcutaneously with ethinyl estradiol 10microgram/kg rat weight. The rats were treated daily for 8 weeks. As expected, ovariectomy has induced oxidative stress as shown by the increment in plasma melondialdehyde (MDA) in OVXgroup. Palm tocotrienols mixture and estrogen therapy were able to prevent oxidative stress by preventing the increasein MDA level after ovariectomy. The protective effect of palm tocotrienols mixture was comparable to estrogen therapy. Palm tocotrienols mixture was as effective as estrogen therapy in alleviating oxidative stress-induced ovariectomy in rat model. Palm tocotrienols mixture supplementation may play a role in offering protection against post menopausal related diseases.