Purification of Apigenin by Preparative TLC  


Baharak Bahmani, Farzaneh Naghibi, Somayeh Esmaeili, Nazli Nader and Mahmoud Mosaddegh


Traditional & Complementary Medicine Exhibition 2007 (TCME 2007), Putra World Trade Centre (PWTC), Kuala Lumpur, Malaysia




Apigenin-Purification-Thin layer chromatography


One of the best active components ofMatricaria chamomillawhich can be used for standardization is apigenin. The objective of this investigation was to develop a chromatography method for the isolation and purification of apigenin. Preparative thin layer chromatography TLC ofMatricaria chamomillamethanolic extract was performed on 20×20 cm plates coated with silica gel 60 PF. TLC with fifteen different solvent systems, NMR and IR spectrophotometery and HPLC analysis were used to confirm the apigenin purity. Different analytical methods were established the purity of obtained apigenin. The method is simple, economical and useful for purification of apigenin inMatricaria chamomillaextract.