Quantitative analysis of 18 β-Glycyrrhetinic Acid in Glycyrrhiza glabra Extract by HPLC


Nazli Nader, Somayeh Esmaeili, Farzaneh Naghibi and Mahmood Mosaddegh


Traditional & Complementary Medicine Exhibition 2007 (TCME 2007), Putra World Trade Centre (PWTC), Kuala Lumpur, Malaysia




Glycyrrhiza glabra, HPLC, ß-Glycyrrhetinic Acid


A high performance liquid chromatography method was studied for determination of 18 β-Glycyrrhetinic Acid inGlycyrrhiza glabra(licorice) extract. The operating condition were C-18 reversed phase column (VP-ODS, (250 x 4.6 mm, 5 mm)) at room temperature, acetonitril/phosphoric acid (3/1) as mobile phase, at ¿ow rate of 0.6 ml/min (0-8 min), 0.4 ml/min (8-20min) and UV detection at 230 nm. The recoveries were 99.60% - 102.81 % with relative standard deviation between 0.01% - 1.58%. The relative standard deviation of the repeatability test was 2.96%. The method is simple, rapid, safe, accurate, economical and useful for standardization of the licorice products.