Antinociceptive Properties of Extracts and Two Flavonoids Isolated from Leaves of Danae racemosa


Nasrin Maleki-Dizaji, Fatemeh Fathiazad and Alireza Garjani


Traditional & Complementary Medicine Exhibition 2007 (TCME 2007), Putra World Trade Centre (PWTC), Kuala Lumpur, Malaysia




Danae racemosa, analgesic, Opioid system


Danae racemosais using as an ornamental plant. To our knowledge, pharmacological properties ofDanae racemosahave not been elucidated to date. In this study the antinociceptive properties of the hydro-methanolic extract (HME) and two flavonoids isolated fromDanae racemosahave been investigated in several models of nociception in rat. The HME ofD. racemosa(100-400 mg/kg, i.p.) produced dose-related and significant inhibition of acetic acid-induced abdominal constriction. In the same range of doses HME produced dose-related inhibition in both phases of formalin-test. Treatment of animals with naloxone (5 mg/kg, i.p.) completely reversed the antinociceptive effect caused by morphine (5 mg/kg, s.c.) and that caused by HME (200 mg/kg, i.p.) when assessed against first phase of the formalin-test, but this effect was less significant for HME in the second phase. Furthermore, when assessed in the hot-plate test, HME (100-400 mg/kg, i.p.)caused significant increase in response latency. HME given daily for to seven consecutive days did develop tolerance to itself, but did not induce cross-tolerance to morphine. These data demonstrate that HME elicited pronounced antinociception against several models of pain. Its actions involve, at least in part, an interaction with opioid system, seeming no to be related with a non-specific peripheral or central depressant actions. Finally, the active principle(s) responsible for the antinociceptive action ofD. racemosais likely related partially to the presence of quercetin and kaempferol.