Effects Of Nicotine Administration And Vitamin E Supplementation On Dynamic Bone Histomorphometric Parameters In Sprague-Dawley Male Rats




The 21st Annual Seminar of the Malaysian Natural Product Society.(Herbal Medicine:Natural Products S ciety), Palm Garden, Putrajaya Malaysia.




Nicotine, vitamin E, alpha-tocopherol, gamma-tocotrienol, bone histomorphometry.


This study assessed the effects of nicotine administration and vitamin E suplementation on dynamic parameters of bone histomorphometry in 30 Sprague-Dawley male rats.  Rats aged three months and weighing between 250-300 g were divided equally into five groups.  Group 1 was the baseline control (BC), which was killed without treatment.  The other 4 groups were the control group (C), the nicotine treated group (N), the alpha-tocopherol treated group (ATF) and the gamma-tocotrienol treated group (GTT).   The N group was treated with nicotine and the C group was treated with normal saline only, for 4 months.  Treatment for the ATP and GTT groups were done in two phases.  For the first two months, the rats were given nicotine and treatment was continued with vitamin E (alpha-tocopherol or gamma-tocotrienol) for the following two months.  Nicotine treatment 7 mg/kg body weight was given by intraperitoneal injection and vitamin E treatment 60mg/kg body weight was given orally for six days a week.  The rats were injected intraperitoneally with calcein 20 mg/kg body weight at day 9 and day 2 before they were killed.  Histomorphometric analysis was done on the metaphyseal region of the trabecular bone of the left femur by using image analyzer interfaced with fluorescence microscope.  The parameters measured were single labeled surface (sLS/BS), double labeled surface (dLS/BS), mineralized surface (MS/BS), mineral appositional rate (MAR) and bone formation rate (BFR/BS).  Nicotine resulted in a significant decrease in the dLS/BS, MS/BS, MAR and the BFR/BS, while an increase in sLS/BS as compared to BC and C group.  Vitamin E treatment reversed all these nicotine effects.  The C group showed no significant changes in all 5 parameters as compared to the BC group.  Furthermore, the GTT group had a significantly higher MAR and BFR values as compared to ATF group.   The effects of GTT and ATF on the other 3 parameters were comparable.  In conclusion, treatment with nicotine 7 mg/kg for four months had affected the dynamic trabecular histomorphometric parameters and treatment with either GTT or ATF at 60 mg/kg for two months reversed the negative effects of nicotine by improving the dynamic trabecular histomorphometric parameters.  GTT was shown to be slightly superior compared to ATF.