Testosterone Effect On Blood Glutathione And Tumor Marker Enzyme In Rat During Hepatocarcinogenesis


Asmah Rahmat, Wan Zurinah Wan Ngah, Zanariah Jarien, Rosnani Ismail, A Rahim Dan & Khalid Abdul Kadir


The Fifteenth Malaysian Biochemical Society In Conference : Focus on Biochemistry in Malaysia in the 1990's, Faculty Of Medicine Universiti Kebangsaan Malaysia, Kuala Lumpur, Malaysia.




hepatocarcinogenesis, testosterone, total blood glutathione, plasma marker enzymes


Sex-differentiated chemical hepatocarcinogenesis in the rat has been reported extensively. This study was to investigate the effect of testosterone in rats induced hepatocarcinogenesis with diethylnitrosamine [DEN) and 2-acetylaminofluo-rene (AAF). The carcinogenic process was followed morphologically and by determining total blood glutathione and two plasma marker enzymes, gamma-glutamyl transpeptidase (GGT) and alkaline phosphatase (ALP). 60 male albino rats, Rattus norwegicus, each weighing (120-160g) were divided into 5 groups according to the different treatments: control, control with 60% ethanol, DEN/AAF, testosterone (1.5mg/rat/day) and mixed treatment with DENI AAF I testosterone. The rats were sacrificed after 4 and 8 weeks. The results obtained indicated no difference morphologically in the livers of control and the different treated rats. Treatment with DEN/AAF caused an increase in GSH levels and both marker enzyme activities when compared to control (p<0.05). Treatment with testosterone and the mixture (DEN/AAF/ testosterone) did not have any effect on blood glutathione when compared to the control but differs significantly (p<0.05) when compared to DEN/AAF treated rats. Treatment with ethanol, DEN/AAF, testosterone and mixture of DEN/AAF/testosterone caused an increase in plasma marker enzymes activities when compared to control after 4 weeks. After 8 weeks, testosterone caused a decrease in GGT and ALP activities and the activities differ significantly when compared to DEN/AAF treated rats. These results suggested that testosterone treatment seemed to decrease the severity of the hepatocarcinogenic process for this method of administration of testosterone.