Biomass Production Of Tongkat Ali (Eurycoma longifolia)


Nallammai Singaram & Chris K.H. Teo, School of Biological Sciences, Universiti Sains Malaysia, Penang, Malaysia.


Trends in Traditional Medicine Research, Proceedings of the International Conference on the Use of Traditional Medicine & Other Natural Products in Health Care




Tongkat Ali, Eurycoma longifolia, biomass production.


Stem sections of tongkat Ali (Eurycoma longifolia) were cultured in Murashige & Skoog (MS) medium. Highest callus production was on MS + 2.0 mg/1 naphthaleneacetic acid (NAA) + 0.1 mg/1 benzyl adenine (BA). Optimum growth of callus was obtained on medium with pH 6.0, adjusted before autoclaving. Cultures grown at 35oC produced the highest amount of biomass. At this culture temperature, light intensity of 610 lux was adequate and gave the highest callus yield. Callus growth was best at 24-hour photoperiod.   The callus produced was yellowish, soft and loose. Successful production of tongkat Ali biomass is the first necessary step towards establishment of cell cultures for the production of secondary metabolites in   vitro.