Optimization of Extraction of Phenolic Antioxidants from Stems of Henna (Lawsonia inermis)


Ho C.W  & Tan M.C.  
Food Science and Nutrition Department and Biotechnology Department,
Faculty of Applied Sciences, University College Sedaya International (UCSI),
No.1, Jalan Menara Gading, UCSI Heights, Cheras 56000, Kuala Lumpur.
Tel: 03-91018880 Fax: 03-91023606 Email: This email address is being protected from spambots. You need JavaScript enabled to view it.




Optimization of Extraction of Phenolic Antioxidants from Stems of Henna (Lawsonia inermis) using Response Surface Methodology (RSM).


Medicinal and Aromatic Plants Seminar (MAPS 2008)


21st October - 22nd October 2008

Place Held

The Legend Hotel, Kuala Lumpur


Henna (Lawsonia inermis) is an Indian medicinal plant used in traditional medicine for the treatment of various diseases, besides its popularity as a natural dye to colour hand and hair. Response surface methodology (RSM) was preformed to optimize the extraction parameters for assessing maximum yield of total phenolic content (TPC) extraction efficiency of henna stems, namely the solvent type, solvent concentration (v/v, %), extraction time(min) and extraction temperature (ºC). A five-level , three-variable central composite rotatable design (CCRD) was then applied to investigate the best possible combination effects of acetone concentration, extraction time and extraction temperature on total phenolic content (TPC) and 2,2-diphenyl-1-picryhydrazyl (DPPH) radical scavenging activity of the extracts. The actual values of the independent variables coded at five levels were selected based on the results of single factor experiments. The determination coefficient R² values were 0.9648 and 0.9665 for TPC and DPPH radical scavenging capacity, respectively and their lack of fit were insignificant at 5% level. All of these indicated a good adequacy of the model proposed to explain the data observed. The optimum conditions for extraction of TPC were found to be at acetone concentration of 47.02%, extraction time of 47.56 min and extraction temperature of 37.30ºC; whereas the highest DPPH radical scavenging capacity was achieved at acetone concentration of 75.81%, extraction time of 26.22 min and extraction temperature of 40.95ºC. Under these optimized conditions, the experimental maximum yield of TPC and DPPH radical scavenging capacity were 5232.39mg GAE/100g DW and 6085.70g TE/100g DW, respectively. These were in close agreement with predicted values. These results indicated that the suitability of the models developed and the succes of RSM in optimizing the extraction conditions of phenolic compounds and antioxidants capacity from henna stems.


Lawsonia inermis (henna); response surface methodology (RSM); antioxidant assays; extraction method; phenolic content


Plenary: Session 3


Think Global, Act Local: A New Paradigm In Malaysian Herbal Industry (Prof. Emeritus Dr. Abdul Latif Ibrahim-UNISEL)