Multi-Steps Infrared Macro-Fingerprinting of Phyllagathis Praetermissa From Two Geographical Origins


Tan H.P. & Chuah C.H. 
Medicinal Plants Programme, Division of Forest Biotechnology, Forest Research Institute Malaysia, 52109 Kepong, Selangor.
Tel: 03-62797341 Fax: 03-62729805 Email: This email address is being protected from spambots. You need JavaScript enabled to view it.

Ling S.K.
Department of Chemistry, University of Malaya, 50603 Kuala Lumpur.




Multi-Steps Infrared Macro-Fingerprinting of Phyllagathis Praetermissa From Two Geographical Origins


Medicinal and Aromatic Plants Seminar (MAPS 2008)


21st October - 22nd October 2008

Place Held

The Legend Hotel, Kuala Lumpur


Phyllagathis praetermissa from two goegraphical  regions of Pasoh Forest Reserve (Negeri Sembilan) and Ampang Forest Reserve (Selangor) in Peninsular Malaysia can be distinguished by using macroscopic infrared (IR) fingerprint method. This method comprises the Fourier transform infrared spectroscopy (FT-IR), second derivative infrared spectroscopy and two-dimensional correlation infrared spectroscopy  (2D-IR) under thermal perturbation. Comparison of the conventional IR spectra, as the first step of identification, showed that the primary difference in samples from two locations was the intensity of characteristic peaks at 1694 cmˉ¹ and 1621 cmˉ¹. There was also a distinctive peak at 1370 cmˉ¹ in samples collected from Ampang Forest Reserve which was not found in samples from Pasoh Forest Reserve. The comparison of the second derivative IR spectra, as the second step of identification, also showed some differences in the range of 1800-450 cmˉ¹. Furthermore, the comparison of 2D-IR correlation spectra based on the positions and intensities of auto-peaks, as the third step of identification, revealed the presence of an auto-peak at 480 cmˉ¹ for samples from Pasoh Forest Research but not samples from Ampang Forest Reserve. On the other hand, an auto-peak at 1510 cmˉ¹ found in samples from Ampang Forest Research was insignificant in samples from Pasoh Forest Reserve. The visual and colourful 2D-IR spectra can futher provide dynamic structural information of chemical components in the samples. Consequently, the multi-steps IR spectroscopy can be an effective, fast and non-destructive method to discriminate P. praetermissa from different geographical origins.


Phyllagathis praetermissa; fourier transform infrared spectroscopy (FT-IR); scond derivative infrared spectroscopy; two-dimensional infrared correlation spectroscopy (2D-IR)


Plenary: Session 3


Think Global, Act Local: A New Paradigm In Malaysian Herbal Industry (Prof. Emeritus Dr. Abdul Latif Ibrahim-UNISEL)