Antioxidative Activity of Selected Species from Malvaceae


Rohana S., Vimala S. & Juliza M.  Medicinal Plants Programme, Forest Research Institute Malaysia, 52109 Kepong, Selangor.  Tel: 603-62797608  Fax: 603-62729805  Email: This email address is being protected from spambots. You need JavaScript enabled to view it.




Antioxidative Activity of Selected Species from Malvaceae


Medicinal and Aromatic Plants Seminar (MAPS 2008)


21st October - 22nd October 2008

Place Held

The Legend Hotel, Kuala Lumpur


Malvaceae or the mallow family is a family of flowering plants containing about 95 genera of herbs, shrubs and trees and perhaps as many as 1,500 species that are further characterized by the presence of stellate hairs and mucilaginous sap. The anti-oxidative activity of the methanol extracts of some selected species from Malvaceae was investigates using DPPH Radical Scavenging Activity and Xanthine/ Xanthine Oxidase Superoxide Scavenging Activity. The main characteristic of an antioxidant is its ability to fight and scavenge free radicals (FR). Reactive FR and oxygen species enter the biological systems from a wide variety of sources. Antioxidant compounds like phenolic acids, polyphenols and flavanoids scavenge FR and thus inhibit the oxidative damage that leads to degenerative diseases. In this study, different plant parts of Hibiscus cannabinus ('kenaf'), H. rosa-sinensis ('bunga raya'), H. sabdariffa ('roselle') and Thespesia populnea ('bebaru') were tested for antioxidant properties using both assays at concentration of 0.25mg/ml. The leaves, stems and twigs of T. populnea and the leaves of H. sabdariffa showed high radical scavenging activity, i.e., 96.6± 1.8, 98.3± 1.7, 87.9.6± 0.6 and 72.7± 0.1%, respectively. In the superoxide scavenging assay, the fruits of H. sabdariffa showed the highest activity, i.e., 94.9± 0.8%. High superoxide scavenging activity was also observed in H.cannabinus leaves  (81.7± 5.0%) and stems (71.2± 1.4%), H. sabdariffa leaves (86.4±0.2%) and stems (89.7± 1.5%) and T.populnea stems (71.1± 1.8%). The rest of the samples showed moderate to low scavenging activity. However, to comprehensively study the different mechanistics aspects of antioxidants, more specific mechanism based methods are needed in addition to these two commonly accepted bioassays.


Antioxidative activity; Malvaceae; DPPH Radical Scavenging; X/XOD Superoxide Scavenging


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