Effect of Aqueous Leaf Extract of Ficus Deltoidea (Mas Cotek) On Induced Acute and Chronic Inflammation in Rats


Khairi H.M., Mamduha A. A., Mom S. & Sulaiman, M.R.
Department of Biomedical Science, Faculty of Medicine and Health Sciences, Universiti Putra Malaysia.
Serdang 43400, Selangor
Tel 03 -89472338 Fax: 03-89436178 Email: This email address is being protected from spambots. You need JavaScript enabled to view it.




Effect of Aqueous Leaf Extract of Ficus Deltoidea (Mas Cotek) On Induced Acute and Chronic Inflammation in Rats


Medicinal and Aromatic Plants Seminar (MAPS 2008)


36th October - 22nd October 2008)

Place Held

The Legend Hotel, Kuala Lumpur


Aqueous leaf extract of Ficus deltoidea (FDAE) was evaluated for its anti-inflammatory properties in induced acute and chronic inflammation in rats. For acute inflammation study, nine groups of ten rats were employed. Two chemical mediators were used to induce paw oedema. bradykinin and histamine (0.1 ml at 1 mg/ml). The animals were pre-treated with 1 ml of 10 mg/kg FDAE, 100 mg/kg FDAE, normal saline, piroxicam (10 mg/kg) and mefenamic acid (10 mg/kg) for each group. The paw was measured using plethysmometer for 5 h. For chronic inflammation study, cotton pellet granuloma test was conducted on six groups of ten rats. Sterile cotton pellets were implanted into the subcutaneous tissue of the dorsal thoracic vertebrae region of the rats and were treated intraperitoneally with 1 ml of normal saline. 25 mg/kg FDAE. 50 mg/kg FDAE, 100 mg/kg FDAE, piroxicam (10 mg/kg) and mefenamic acid (10 mg/kg) for each group. Rats were then sacrificed and the pellets were removed to obtain both wet and dry weights. Results were expressed as meanĀ±S.E.M. and analyzed statistically using the ANOVA followed by Duncan post hoc test. Results were considered significant if p<0.05. Both experiments demonstrated significant difference (p<O.05) when compared to the respective negative control groups. In the mediator- induced paw oedema test. 100mg/kg of aqueous extract showed higher inhibition compared to 10 mg/kg. Nevertheless, the extract showed similar effectiveness to mefenamic acid. In the cotton pellet granuloma test, the intermediate dose (50 mg/kg) proved to have the highest transudation inhibition compared to the other dosages. In addition, the effectiveness of the extract was better than piroxicam and mefenamic acid. Nevertheless, as an anti-proliferative agent, piroxicam had the highest inhibition percentage while the extract was better than mefenamic acid. As a conclusion, aqueous leaf extract of F. deltoidea possessed the abilities to inhibit the activity of bradykinin and histamine during acute inflammatory process and to reduce transudation during chronic inflammation.


Ficus deltoidea; mas cotek; leaf extract; anti-inflammatory activity; acute and chronic Inflammation study


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