Constituents of Carica Papaya Leaves and Their Antiplasmodial Activity


Adlin A., Noor Rain A., Badrul Amini A.R., Noor Shakila I. and Zakiah I.
Herbal Medicine Research Centre, Institute for Medical Research, Jalan Pahang, 50588 Kuala Lumpur
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Constituents of Carica Papaya Leaves and Their Antiplasmodial Activity


Medicinal and Aromatic Plants Seminar (MAPS 2010)


3rd August - 4th August (2010)

Place Held

Forest Research Institute Malaysia(FRIM)


The juice from the green leaves of Carica papaya is recommended by traditional practitioners for the treatment of fever. Multiple chromatographic procedures and SYBR Green I assay were performed to identify the major constituents of the ethno-based preparation and to evaluate the antiplasmodial activity of the compounds. The structures of the constituents were elucidated by NMR spectroscopy (1H, 13C, COSY, HSQC and HMBC), mass spectrometry and HPLC-DAD analysis. Extract and fractions from the juice leaves exhibited good anti-plasmodial activity against the chloroquine resistant strain P. falciparum K1 and the local sensitive strain Gombak A, with IC50 ranging from 0.32 to 3.89 µg/mL. Through bioassay guided fractionation, three major flavonol glycosides; manghaslin (1), clitorin (2) and rutin (3) were identified and exhibited good anti-plasmodial activity against Gombak A strain with 3 being the most active (lC50=0.918 ± 0.574 µM). Only 1 showed good anti-malarial activity against K1 strain. The results provided a scientific basis on the ethnomedical use of C. papaya.


Carica papaya; flavonol glycoside; SYBR Green I; anti-plasmodia)


Session 2: oral 12


Harnessing the Tropical heritage: Recent Advances in R&D and commercialization