Selection Of Cell Lines For Cell Suspension Culture Of Artemisia Annua L. Of Vietnam Origin


Ch’na S.J., Yahaya Z.S., Tran V.M., Boey P.L and Chan L.K.
School of Biological Sciences, Universiti Sains Malaysia, 11800 Minden, Penang, Malaysia School of Chemical Science, Universiti Sains Malaysia, 11800 Penang, Malaysia
Institute of Tropical Biology, Vietnam Academy of Science and Technology, Ho Chi Minh City. Vietnam
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Selection Of Cell Lines For Cell Suspension Culture Of Artemisia Annua L. Of Vietnam Origin


Medicinal and Aromatic Plants Seminar (MAPS 2010)


3rd August - 4th August (2010)

Place Held

Forest Research Institute Malaysia(FRIM)


Artemisia annua L. is the source of artemisinin which is the most efficient anti-malarial drug against the multidrug resistant strains of Plasmodium. Due to the environmental and economical factor, in vitro cell suspension culture technique was reported as one of the alternative ways for mass production of this compound. Selection of the starting material is important for the establishment of cell suspension culture. Cell lines were produced from in vitro leaf explants of three A. annua clones (TC1, TC2 and Hi) which germinated from the seeds collected from two different geographical locations in Vietnam. The growth index of the cell lines were recorded every four weeks by sub-culturing 0.5g of the callus into fresh medium for 13 subculture cycles, The cell lines were categorized into fast, intermediate and slow growing based on the growth index of the callus. The cell lines with consistent growth index were selected for the initiation of cell suspension culture of A. annua.


Anti-malarial; artemisinin; cell Lines; callus culture; growth index


Poster Abstracts: P09


Harnessing the Tropical heritage: Recent Advances in R&D and commercialization