Optimization of Solid-Liquid Extraction of Betel Leaves (Piper Betle L.)


Pin K.Y, Luqman Chuah A., Abdull Rashih A., Rasadah M.A., Law C.L. & Choong T.S.Y. Medicinal Plants Division, Forest Research Institute Malaysia, 52109 Kepong, Selangor Department of Chemical and Environmental Engineering, University Putra Malaysia, 43400 UPM Serdang, Selangor Faculty of Engineering and Computer Science, University of Nottingham, 43500 Semen yih, Selangor 
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Optimization of Solid-Liquid Extraction of Betel Leaves (Piper Betle L.)


Medicinal and Aromatic Plants Seminar (MAPS 2010)


3rd August - 4th August (2010)

Place Held

Forest Research Institute Malaysia(FRIM)


Betel (Piper betle L,) is a medicinal plant with multiple traditional applications which originated from Peninsular Malaysia. It is locally known as Sirih and reported to possess various beneficial bioactivities including anticarcinogenic, anti-inflammatory, antioxidant, and antimicrobial. The study was aimed to determine the optimum solid-liquid extraction (SLE) parameters including extraction temperature (T) and ratio of solvent to solid (R) for betel leaves. The optimization was carried out by considering the quality of extract and yield of SLE. The quality of extract was evaluated by comparing the contents of its active phytochemicals namely hydroxychavicol and eugenol which were quantified with High Performance Liquid Chromatography (HPLC). The effects of T and R on the yield of SLE were studied using response surface methodology (RSM). The HPLC results indicated that the concentration of HC in the extracts increased with the extraction temperature. The percentage of increase dropped after 60°C because the system was approaching the equilibrium in which saturation of HC happened. On the other hand, the concentration of EU increased when the extraction temperature was raised from 25 to 60°C but decreased as higher temperatures, 70 and 80°C, were applied. A second-order polynomial model with regression coefficient, R2, of 0.94 was solved using RSM for predicting of yield of SLE. The analysis showed that temperature exhibited greater effect on the yield compared to ratio of solvent to solid. The optimum temperature and ratio of solvent to solid were determined as 60ºC and 30:1 (ml:g).


Piper betle L.; Optimization; Solid-liquid Extraction; Response Surface Methodology.


Poster Abstracts: P40


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