The aim of extract standardization is to yield a standardized extract. Standardized extract is an active constituents isolated from herbs and concentrated to a specific, guaranteed quality product and expressed as percentage.
Extract Standardization
| Steps | Aims | |
| Medicinal plant discovery | Botanical study | Morphological investigation of medicinal plant including macroscopic and microscopic studies. Literature review based on monograph or pharmacopoeia. Authentication of medicinal plant. |
| Extract preparation | Phytochemical investigation | Determination of raw material preparation method. Standardization of extraction method. Composition analysis. Characterization of purity, impurities and contaminants. Determination of marker compound or active constituent. |
| Extract fractionation | Identification of marker compound or active constituent | Analysis of marker compound or active constituent for preliminary identification. Structural elucidation of compounds in extract preparation. |
| Chemical characterization | Physico chemical properties | Chemical identification of marker compund or active constituents by fingerprinting (chromatogram, spectroscopic etc.) Quantification of marker compound or active constituent. Physico chemical property measurements of marker compound/active constituent and extract. Physical and chemical characterization of extract. Structural elucidation of marker compound or active constituent. Standardization of herbal extract. |
| Primary pharmacodynamic study | Biological characterization | Screening of pharmacology or therapeutic effect. |
