The aim of extract standardization is to yield a standardized extract. Standardized extract is an active constituents isolated from herbs and concentrated to a specific, guaranteed quality product and expressed as percentage.
|Medicinal plant discovery||Botanical study||Morphological investigation of medicinal plant including macroscopic and microscopic studies. Literature review based on monograph or pharmacopoeia. Authentication of medicinal plant.|
|Extract preparation||Phytochemical investigation||Determination of raw material preparation method. Standardization of extraction method. Composition analysis. Characterization of purity, impurities and contaminants. Determination of marker compound or active constituent.|
|Extract fractionation||Identification of marker compound or active constituent||Analysis of marker compound or active constituent for preliminary identification. Structural elucidation of compounds in extract preparation.|
|Chemical characterization||Physico chemical properties||Chemical identification of marker compund or active constituents by fingerprinting (chromatogram, spectroscopic etc.) Quantification of marker compound or active constituent. Physico chemical property measurements of marker compound/active constituent and extract. Physical and chemical characterization of extract. Structural elucidation of marker compound or active constituent. Standardization of herbal extract.|
|Primary pharmacodynamic study||Biological characterization||Screening of pharmacology or therapeutic effect.|