In Vitro Screening of DPPH Free Radical Scavenging and Antimicrobial Compounds fromZizyphus mauritianaLam

Author

HABSAH MOHAMAD, NORAZIDA DAUD, ZALILAWATI MAT RASHID, MOHD NORDIN HJ LAJIS & MOHAMAD HUSSIN ZAIN

Proceeding

The 21st Annual Seminar of the Malaysian Natural Product Society.(Herbal Medicine:Natural Products S ciety), Palm Garden, Putrajaya Malaysia.

Date

22/11/2005

Keyword

Zizyphus mauritania Lam, betulinic acid derivatives, antioxidant, DPPH free radical scavenging, antimicrobial.

Abstract

Zizyphus mauritianaLam  was subjected to thin layer chromatography, column chromatography  and chromatotron to yield two new betulinic acid derivatives,  ZM1 and ZM2. The structures of the compounds were analysed by Fourier Transform Infrared spectrometer (FT-IR), ultraviolet and visible (UV-Vis) spectrophotometer, gas chromatography-Mass spectrometer (GC-MS) and 1HNMR.. The antioxidant activity of the compounds was determined by using the DPPH free radical scavenging assay. Both compounds displayed DPPH free radical scavenging activity. ZM2 possessed mild antimicrobial activity.