Sinensetin and Rosmaranic Acid Contents In Different Growth Stages of Orthosiphon Stamineus (Misai Kucing)

Author

Zamree MS., Hada Masayu I.D., Mohd Shahidan M.A., Pin K.Y., Noor Rasyila M.N., Khairul K.A.K., Noor Syaheera M.Y., Masitah M.T., Mohd Faizal K. & Rasadah M.A.
Medicinal Plants Programme, Forest Biotechnology Division. Forest Research Institute Malaysia. 52109
Kepong, Selangor
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Language

English

Title

Sinensetin and Rosmaranic Acid Contents In Different Growth Stages of Orthosiphon Stamineus (Misai Kucing)

Proceeding

Medicinal and Aromatic Plants Seminar (MAPS 2008)

Date

21st October - 22nd October 2008

Place Held

The Legend Hotel, Kuala Lumpur

Abstract

Sinensetin (SEN) and rosmarinic acid (RA) in leaf and stem parts of Orthosiphon stamineus (OS) at different growth stages (1, 2 and 3 months), were quantified in order to determine the best time for harvesting purposes. An HPLC method was developed for quantification of SEN and RA. The compounds were separated using a reversed phase C18 Phenomenex column under gradient condition with methanol:Water:Phosphoric acid (v/v/v) as mobile phase. The results showed that RA was the main component found in OS which was ranging from 7.13 to 7.98 ppm in leaf and from 1.37 to 1.63 ppm in stem pan of OS. Concentrations of SEN ranged from 0.56 to 0.70 ppm in leaf and 0.53 to 0.54 ppm in stem part of OS. The results also revealed that there is no significant difference in the RA concentration determined in every growth stage studied. However, SEN concentration in month 2 and 3 were significantly higher compared to first month. As a conclusion, OS leaf contains higher concentration of RA and SEN compared to its stem and the best time to harvest OS is two months after growing.

Keywords

Orthosiphon stamineus; sinensetin; rosmarinic acid; harvesting

Session

Poster Abstracts

Topic

Think Global, Act Local: A New Paradigm In Malaysian Herbal Industry (Prof. Emeritus Dr. Abdul Latif Ibrahim-UNISEL)