Comparative Study on Culture Media for Establishment of Cell Suspension Culture of Artemisia Annua L.

Author

Lo K.Y., Sreeramanan S., Tran V.M., Boey R.L. and Chan L.K.,
School of Biological Sciences, Universiti Sains Malaysia, 11800 Minden, Penang, Malaysia
School of Chemical Sciences, Universiti Sains Malaysia, 11800 Penang, Malaysia
lnstitute of Tropical Biology, Vietnam Academy of Science and Technology, Ho Chi Minh City, Vietnam
Tel: 04-6533520 E-mail: This email address is being protected from spambots. You need JavaScript enabled to view it.

Language

English

Title

Comparative Study on Culture Media for Establishment of Cell Suspension Culture of Artemisia Annua L.

Proceeding

Medicinal and Aromatic Plants Seminar (MAPS 2010)

Date

3rd August - 4th August 2010

Place Held

Forest Research Institute Malaysia(FRIM)

Abstract

Artemisinin isolated from the aerial parts of Artemisia annua plants have been proven to be effective for the treatment of both drug resistant and cerebral malarial parasites. Although the production of artemisinin by biological and chemical means is possible, it is not economically feasible. Therefore, enhanced production of artemisinin via in vitro cell culture technique is highly desirable. Friable leaf-derived callus of A. annua was transferred from the solid medium into nine different liquid media containing different chemical compositions to determine the suitable medium for the preparation of cell suspension culture of A. annua. Only two media were found to be optimum for A. annua cell growth, i.e., MS medium supplemented with 0.5mg/L 2, 4-D and MS medium added with 0.5 mg/L BA, 0.5mg/L NAA and 0.5g/L casein hydrolysate. Cells cultured in these two media were light green and green in colour, respectively. After eight subculture cycles of 14 day interval, cells in MS medium supplemented with 0.5mg/L 2, 4-D turned blackish brown, and the cell growth index decreased after each subculture cycle, indicating occurrence of cell death. Whereas, cells cultured in MS medium + 0.5 mg/L BA + 0.5mg/L NAA + O.5g/L casein hydrolysate were green in colour and produced consistent cell biomass with growth index of 11.2—i 1.8 at each subculture cycle.

Keywords

Artemisinin; culture medium; growth index; casein hydrolysate

Session

Session 1: Oral 4

Topic

Harnessing the Tropical heritage: Recent Advances in R&D and commercialization