Anti-Metastatic Properties of Xanthorrhizol Towards Highly Metastatic Human Breast Carcinoma Cell Line, MDAM B-231

Author

Fariza Juliana N., Yew H.C., Azimahtol Hawarlah L.P., Noor Rain A. and Zakiah I. Bioassay Unit, Herbal Medicine Research Center, Institute for Medical Research, 50588
Jalan Pahang. Kuala Lumpur
School of Biosciences and Biotechnology. Faculty of Science and Technology,
National University of Malaysia, 43600 UKM Bangi, Selangor

Language

English

Title

Anti-Metastatic Properties of  Xanthorrhizol Towards Highly Metastatic Human Breast Carcinoma Cell Line, MDAM B-231

Proceeding

Medicinal and Aromatic Plants Seminar (MAPS 2010)

Date

3rd August - 4th August (2010)

Place Held

Forest Research Institute Malaysia(FRIM)

Abstract

Xanthorrhizol is a natural sesquiterpenoid compound derived from the plant Curcuma xanthorrhiza D. Dietr. locally known as “temu lawak”. Xanthorrhizol has apoptotic effect towards human breast carcinoma cell line, MDA-MB-231. In this study, we further evaluated the anti-metastatic activities of xanthorrhizol in vitro. Adhesion, migration and invasion were the essential steps in metastasis cascade. The effects of xanthorrhizol on metastatic characteristic of human breast carcinoma cell line. MDA-MB-231 were determined by adhesion, migration and invasion assays. Cells were treated with xanthorrhizol at various concentrations viz. 0, 5, 10, 15, 20 and 25 pg/ml for 24 h. For adhesion assay, treated cells were plated into laminin, gelatin, collagen I, collagen IV, fibronectin or poly-D-Iysin coated plates and stained with 0.1% crystal violet after 24 h incubation. Migration and invasion assays were done by using BD transwell but the invasion well was pie-coated with matrigel that acted as extracellular matrix barrier. The expression of matrix metalloproteinase-9 was measured by gelatin zymography. Our results showed that xanthorrhizol inhibited the adhesion, migration and invasion ability of the highly metastatic MDA-MB-231 cells. The concentration of xanthorrhizol to inhibit 50% MDA-MB-231 cell adhesion to the substrates of fibronectin, poly-D-Iysin, collagen I, collagen IV, laminin and gelatin were 11.399±1.006, 10.595±1.572, 12.860±0.926, 12.524±0.508, 10.348±1.623 and 8.315±2.881 pg/ml, respectively. Fifty percent of the MDA-MB-231 cell migration was inhibited at the concentration of 17.125±5.540 pg/ml. Xanthorrhiizol at concentration 15.621±1.688 µg/ml inhibited 50% of MDA-MB-231 cell invasion. This effect was associated with the decrease in the MMP-9 protein expression. In condusion, xanthorrhizol exhibited anti-metastastic effects towards highly metastatic human breast carcinoma cell tine, MDA-MB-231. Thus, it is worth to be further evaluated for its use as anti-metastatic agent for breast carcinoma.

Keywords

Xanthorrhizol; anti-metastasis; human breast carcinoma cell; MMP-9 protein

Session

Session 2: Oral 6

Topic

Harnessing the Tropical heritage: Recent Advances in R&D and commercialization