Qualitative Phytochemical Analysis of Psidium Guajava L. Leaf Extracts and their Inhibitory Effects on Human Osteosarcoma Cells

Author

Nor Farhana., Dasuki MS. and Azman P.K.M.
School of Health Sciences
School of Dental Sciences, Universiti Sains Malaysia, Health Campus, 16150 Kubang
Kerian, Kelantan
Tel: 609-097677581 Fax: 609-097677515 Email: This email address is being protected from spambots. You need JavaScript enabled to view it.

Language

English

Title

Qualitative Phytochemical Analysis of Psidium Guajava L. Leaf Extracts and their Inhibitory Effects on Human Osteosarcoma Cells

Proceeding

Medicinal and Aromatic Plants Seminar (MAPS 2010)

Date

3rd August - 4th August (2010)

Place Held

Forest Research Institute Malaysia(FRIM)

Abstract

The world of modern medicine is now beginning to accept the use of botanicals once they are scientifically validated. One example is the Psidium guajava L. or commonly known as guava. The leaves of P.guajava are very well known for their vast traditional uses and purposes. One of the areas of interest is the potential chemo-preventive activities including the treatment of malignant cancer that develop in the bone (osteosarcoma or osteogenic sarcoma). The first objective of this study was to perform qualitative phytochemical evaluation on three types of leaf extracts of P. guajava including methanol, petroleum ether and water extracts. Second objective was to determine the inhibition concentration (lC50) of the extract on osteosarcoma cell line, CRL 1543. Leaves of P guajava from one of the local household in Kelantan were collected, processed and extracted using the three solvents. Qualitative phytochemical analysis on the P. guajava extracts showed that the methanol and water extracts contained alkaloids, tannins and saponins. The petroleum ether extract on the other hand contained only alkaloids and terpenoids. The methanol extract showed significant growth reduction activity in CRL 1543 with an lC50, concentration of approximately 50 ug/ml. The result of the reducing cell numbers was supported by the presence of apoptotic features observed in the culture. The findings of this study may be used for future research in developing new anticancer agents derived from the guava leaf extract for the osteosarcoma patients. However, further in-depth studies are needed to confirm the exact cell death mechanism initiated by the P.guajava leaf extract on human osteosarcoma cell line.

Keywords

Psidium guajava L.; phytochemical analysis; methanol extract; osteosarcoma

Session

Poster Abstracts: P29

Topic

Harnessing the Tropical heritage: Recent Advances in R&D and commercialization