Hepatoprotective Effect of Fumaria parviflora L. Extract Against Carbon Tetrachloride Induced Toxicity in Rats


Hossein Niknahad, Akram Jamshidzadeh, Syyed Mohammad and Ahmadi


Traditional & Complementary Medicine Exhibition 2007 (TCME 2007)




Fumaria parviflora, carbon tetrachloride, hepatotoxicity


Fumaria parvifloraL. has been used in the Iranian traditional medicine for treatment of hepatic diseases. We studied the effects of the extracts of the plant against hepatotoxicity of CCl4 in rats. Hydroalcoholic extract of the whole plant was preparedand then hexane, ethylacetate, methanolic and ethylacetate-methanol fractions were separated by column chromatography. Rats received different doses (50-500 mg/kg) of the total extract or each fraction by oral rout for three consecutive days before the i.p. injection of 3 ml/kg CCl4. Twenty four hours later, the rats were sacrificed and blood and liver samples werecollected for biochemical and histopathological studies. The results showed that total hydroalcoholic extract and the hexane fractions ofF. parviflorahad the best hepatoprotective effect against CCl4 induced toxicity. They decreased the level of serum SGOT, SGPT and billirubin, and prevented histopathological liver damages. Lipid peroxidation and GSH depletion in the liver induced by CCl4 were also prevented by the extracts. The other extracts with low doses (50-100 mg/kg) were also hepatoprotective, however, higher doses of different fractions, did not prevent hepatotoxicity of CCl4. The results of this study support the traditional believes on hepatoprotective effect ofF. parvifloraL. Hepatoprotectivetive components of this plant,possibly antioxidants or radical scavengers, are best extracted in the hexane fraction.