Longilactone C19 from Eurycoma longifolia, Induces Apoptosis and Activates Caspases in MCF-7 Human Breast Cancer Cells


Shamsul Muhamad, Azimahtol Hawariah Lope Pihie, Noor Rain Abdullah and Zakiah Ismail


Traditional & Complementary Medicine Exhibition 2007 (TCME 2007), Putra World Trade Centre (PWTC), Kuala Lumpur, Malaysia




Eurycoma longifolia, apoptosis, breast cancer


The objective of the present study was to evaluate the effect of longilactone C19, a quassinoid isolates from root ofEurycoma longifolia, which induces apoptosis in MCF-7 human breast cancer cells, on two pathways leading to apoptosis. These pathways are classified based on the localization of the initiated signal, extrinsic and intrinsic pathways. Extrinsic and intrinsic pathways are catalyzed by caspases-8 and caspase-7. The results indicate that longilactone C19-induced apoptosison MCF-7 through activation of caspase-8 and downstream protein i.e. caspase-7 and PARP but not caspase-9. It concluded that longilactone C19may induce apoptosis through extrinsic pathway.