Genotoxicity of Zerumbone in Human Peripheral Blood Lymphocytes Evaluated by Cytogenetic Tests


Adel Al-Zubairi, Ahmad Bustamam Hj Abdul, Siddig Ibrahim, M.N. Somchit2


Traditional & Complementary Medicine Exhibition 2007 (TCME 2007),Putra World Trade Centre (PWTC), Kuala Lumpur, Malaysia




Zerumbone; chromosome aberrations; Micronucleus; Human lymphocytes


A natural compound, zerumbone was extracted, isolated and purified from the rhizomes of edible plantZingiber zerumbetusing methanol extraction and column chromatography method. The purified zerumbone has been investigated for its anti-cancer properties on human cervical cancer cell line (HeLa) and compared to cisplatin, the current commercial drug used to treat cervical cancer in human. In this study we investigated the genotoxicity of zerumbone in normal human peripheral blood lymphocytes compared to cisplatin using two cytogenetic end points, namely chromosome aberration assay (CA) and micronucleus test (MN). Peripheral blood lymphocytes obtained from healthy donors were incubated with different concentrations of zerumbone and cisplatin for 24 hours. Mitomycin-C was used as a positive control. The results of the chromosome aberrations assay and micronucleus test showed that zerumbone induces chromosome aberration: gaps and breaks as well as dicentrics.