Effect Of Tocotrienols On Total Glutathione And Glutathione S-Transferase Activities In he Kidneys Of Rats Treated with 2-Acetylaminoflourene


Wan Zurinah Wan Ngah, Rusli Ibrahim, Abdul Rahim Dan, Nor Aripln Shamaan & Khalid BAK.


The Fifteenth Malaysian Biochemical Society In Conference : Focus on Biochemistry in Malaysia in the 1990's, Faculty Of Medicine Universiti Kebangsaan Malaysia, Kuala Lumpur, Malaysia.




tocotrieno, total glutathione (GSH, glutathione S-transferase activities (GSH-Tr)


The effect of tocotrienol (a form of vitamin E present in large quantities in palm oil) on rat kidneys treated with2-acetylaminofluorene (AAF) was investigated by determination of total glutathione (GSH) and glutathione S-transferase activities (GSH-Tr) using both CDNB and DCNB as substrates. 96 male rats Rattus norwegicus weighing 170-200g each were divided into 4 groups according to the different treatments: control, tocotrienol AAF and combined AAF and tocotrienol The rats were sacrificed after 3,8,12 and 20 weeks of treatment and the enzyme activities were measured. Administration of AAF caused an increase in total GSH content and this increase was significant after 20 weeks of treatment (p<0.05) when compared to control rats. Rats receiving the mixture of tocotrienol and AAF also showed an increase in total GSH level although the increase was not significant when compared to the control. However, there was a significant difference in total GSH content when AAF only treated rats were compared to the mixture treated rats. GSH-Tr activities were also increased with treatment with AAF and the mixture although the increase was more obvious with DCNB as the substrate. The increase in enzyme activities were significant (p<0.05) after 8, 12 and 20 weeks (DCNB) and112 and 20 weeks (CDNB) of treatment. Tocotrienol on its own had no effect on the total GSH level and GSH-Tr activities using both CDNB and DCNB as substrates.