Articles

EFFECT OF MGN-3 ON HUMAN NATURAL KILLER CELL ACTIVITY AND INTERFERON- y SYNTHESIS IN VITRO.

Author

Ghoneum M, Namatalia G, Kim C.

Date

6/1996

Journal

FASEB JOURNAL

Abstract

MGN-3 is an extract of arabinoxylane from rice bran that has been enzymatically treated with an extract from Hyphomycetes mycelia. In this study we investigated the effect. MGN-3 on natural killer (NK) cell activity and interferon-y (IFN-y) synthesis by peripheral blood mononuclear cells (MNC). MNC was prepared from peripheral blood of healthy individuals and incubated with various concentrations of MGN-3 for 16 hrs., and then NK cell activity was measured by 4 hr. Cr-release assay using K56 Tumor cells as target NK activity was significantly enhanced (2-5 fold) by treatment with MGN-3 at 0-100 u g/ml. In an attempt to investigate the mechanism by which MGN-3 enhances NK activity, we examined the effect of MGN-3 on IFN-y production by MNC. Culture supernatants of the cells incubated with MGN-3 were collected and analyzed for INF-y synthesis by ELISA. INF-y production was increased >2 fold. We concluded that MGN-3 is a potent biological response modifier (BRM) and may be useful in immunotherapy of cancer.